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Image Search Results
Journal: Cell cycle (Georgetown, Tex.)
Article Title: RHAMM is differentially expressed in the cell cycle and downregulated by the tumor suppressor p53.
doi: 10.4161/cc.7.21.7014
Figure Lengend Snippet: Figure 4. Doxorubicin treat- ment leads to RHAMM mRNA downregulation in cells with wild-type p53. Human colon carcinoma cells HCT116 with wild- type p53 (HCT116 p53+/+) and with targeted dele- tions in both p53 alleles (HCT116 p53-/-) as well as the hepatocarcinoma cell lines HepG2 (wild-type p53) and Hep3B (p53- negative) were treated with doxorubicin for 24 h or 48 h. RHAMM mRNA levels were measured by real- time RT-PCR. (A) RHAMM mRNA levels in HCT116 (mean ± SD, n = 3). (B) RHAMM mRNA levels in HepG2 and Hep3B (mean ± SD, n = 2). **p < 0.05; ***p < 0.01. (C) Cell cycle distribution of doxorubicin- treated HCT116 cells was analyzed by FACS after propidium iodide staining.
Article Snippet: HepG2,
Techniques: Quantitative RT-PCR, Staining